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FVB.Cg-Gt(ROSA)26Sortm1(CAG-lacZ, -EGFP)Glh/J
Cat. No.: CEMM-07250222
Common Name: R26NZG
The R26NZG targeting vector contains a loxP-flanked PGK-Neo-4x polyA STOP cassette, FRT-flanked a nuclear localized β-galactosidase (nlslacZ)-3x polyA, and enhanced green fluorescent protein (EGFP)-1x polyA. These mice may be useful as a Cre mediated reporter or a FLP reporter when expressed in cells/tissues where the expression patterns of the individual promoters driving CRE recombinase and FLP recombinase overlap.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
lacZ, beta-galactosidase, E. coli; GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
Cre excision of the stop signal results in LacZ expression in Cre-expressing tissues, while removal of the stop signal and Flp-mediated excision of the LacZ results in of expression of EGFP in Cre-expressing tissues.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6J)F1
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| Molecular Note |
A vector containing a CAG promoter driving expression of a floxed neo/STOP cassette, FRT flanked lacZ gene with 3 polyadenylation sequences (secondary STOP cassette), and an EGFP gene was inserted into the locus. Expression of lacZ requires Cre mediated removal of the primary STOP cassette whereas expression of EGFP requires Cre mediated removal of the primary stop cassette and flp mediated removal of the secondary STOP cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
FVB/NJ
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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