C57BL/6-Tg(Cck-cre)CKres/J

Cat. No.: CEMM-07250211

A minimal Cck (cholecystokinin) promoter drives expression of Cre in this transgenic strain. These mice express Cre at high levels in the brain cortex in a pattern that is qualitatively similar to that of the wildtype Cck gene. This strain may be useful for various psychiatric and neuroscience studies of this neuropeptide promoter.

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Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(Cck-cre)CKres show more close
Allele Name	transgene insertion C show more close
Allele Attributes	Recombinase-expressing show more close
Gene Symbol	Tg(Cck-cre)CKres show more close
Gene Name	transgene insertion C show more close
Chromosome	UN show more close
Expressed Genes	Cre, Cre recombinase, bacteriophage P1; RFP, Red Fluorescent Protein, coral show more close
MGI Accession ID	4438218 show more close
Site of Expression	High levels of Cre expression in the cortex. show more close
Strain of Origin	C57BL/6 show more close
Molecular Note	The coding sequence for Cre recombinase, followed by an IRES and the DsRed2 sequence was placed downstream of a 3kb cholecystokinin (CCK) promoter. The construct was linearized, purified, and microinjected into the pronuclei of one-cell C57BL/6 embryos. Lines A, B, and C were established and analyzed. None of the three lines showed any observable DsRed fluorescence. Line C showed high levels of Cre expression in the cortex, in a manner qualitatively similar to observed patterns of endogenous Cck mRNA expression, but showed almost no Cre-mediated LacZ expression in any subcortical regions. show more close

HUSBANDRY
Suggested Controls	C57BL/6J show more close
Breeding Considerations	When maintained as a live colony, hemizygotes may be bred. show more close

For Research Use Only.