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B6(Cg)-Dbpem1Drw Tyrc-2J/J

Cat. No.: CEMM-07251082

Common Name: DbpKI;tyr
Albino DbpKI; tyr conditional circadian reporter mice express firefly luciferase upon Cre recombinase-mediated excision of a loxP-flanked destabilized GFP (d2EGFP) cassette. This CRISPR/Cas9-generated knock-in allele enables the in vivo and ex vivo assessment of bioluminescent rhythms in specific cellular populations.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Tyrc-2J
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Allele Name
albino 2
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Allele Attributes
Null/Knockout
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Gene Symbol
Tyr
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Gene Name
tyrosinase
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Chromosome
7
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MGI Accession ID
1855985
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Site of Expression
Expressed in melanosomes
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Strain of Origin
B6.Cg-Tyrp1b Hps1ep
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Molecular Note
This mutation has a G-to-T base change at coding nucleotide 230 resulting in an amino acid change from arginine to leucine at residue 77 (p.R77L) which lies in the highly conserved DDRE sequence. Nucleotide 230 is at the alternative 5' splice donor site for exon 1 and this allele does not produce the 1a or 1b subset of tyrosinase transcripts but does produce a significant inCrease in 1c and 1d transcripts. Western blots of homozygous mutant skin extracts demostrate the nearly complete absence of the broad 76-84 kDa band of glycosylated wild-type tyrosinase. No tyrosinase activity was found in hairbulb extracts from homozygous mice.
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HUSBANDRY
Suggested Controls
B6(Cg)-Tyrc-2J/J
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Breeding Considerations
Floxed compound heterozygotes and homozygotes are viable and fertile.
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Breeding Strategy
HET X HET
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For Research Use Only.
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