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B6.Cg-Col1a1tm1(CAG-Sirt1)Dsin/Mmjax
Cat. No.: CEMM-07250201
The SIRT1STOP (or SIRT1-Tg flox-STOP-flox) targeted allele has a floxed-STOP cassette and mouse sirtuin 1 (Sirt1) cDNA sequence inserted into the 3' UTR of the collagen A1 (Col1a1) locus. When bred to mice that express Cre recombinase, offspring will have the floxed-STOP cassette deleted in the cre-expressing tissue(s), resulting in Sirt1 overexpression in Col1a1-expressing cells. These SIRT1STOP (or SIRT1-Tg flox-STOP-flox) mice may be useful for generating conditional mutations for studying the role of sirtuin NAD+-deacetylases in aging, cancer, cellular stress resistance, genomic stability, IGF1 pathways, autoimmunity, inflammation, T cell tolerance, energy metabolism, neurodegeneration, insulin resistance, and diabetes.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Col1a1tm1(CAG-Sirt1)Dsin
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Inserted expressed sequence
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| Gene Symbol |
Col1a1
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| Gene Name |
collagen
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| Chromosome |
11
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| Expressed Genes |
Sirt1, sirtuin 1, mouse, laboratory
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| MGI Accession ID | show more close |
| Strain of Origin |
(C57BL/6 x 129S4/SvJae)F1
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| Molecular Note |
The SIRT1-Tg flox-STOP-flox construct was designed to insert (from 5' to 3') an FRT site, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector), a loxP-flanked transcriptional STOP cassette, the mouse sirtuin 1 (Sirt1) cDNA sequence, and a PGK-hygro-polyA sequence (with a second FRT site between the PGK and hygro) into the 3' UTR of the mouse collagen A1 (Col1a1) locus.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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