Online Inquiry
B6.Cg-Tg(CAG-GCaMP2)2Mik/J
Cat. No.: CEMM-07250570
Common Name: CHROMus line pCAGGS-GCaMP2
pCAGGS-GCaMP2 transgenic mice are a fluorescent calcium indicator strain that exhibits high calcium-sensor expression in widespread cells/tissues. Upon GCaMP2 binding to calcium, increased EGFP fluorescence is observed. The entire transgene is flanked with loxP sites.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(CAG-GCaMP2)2Mik
show more
close
|
| Allele Name |
transgene insertion 2
show more
close
|
| Allele Attributes |
Reporter
show more
close
|
| Gene Symbol |
Tg(CAG-GCaMP2)2Mik
show more
close
|
| Gene Name |
transgene insertion 2
show more
close
|
| Chromosome |
UN
show more
close
|
| Expressed Genes |
GCaMP, Genetically encoded calcium indicator
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
EGFP expression is widespread.
show more
close
|
| Strain of Origin |
(C57BL/6 x DBA/2)F2
show more
close
|
| Molecular Note |
This 3.7 kbp transgene has a loxP site, the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), the GCaMP2 sequence (described below), a polyadenylation signal and a second loxP site. Founder line 2 was identified with high sensor expression.The genetically encoded calcium indicator GCaMP2 is a calcium-sensing molecule composed of a 35 aa polyHis plasmid leader sequence (RSET; essential for thermal stability), a 13 residue peptide of chicken smooth muscle myosin light chain kinase (M13), a circularly permutated EGFP (cpEGFP; aa 149-238 followed by aa 1-144 [with mutations listed below]), and a rat calmodulin DNA fragment (CaM; aa 2-148). The cpEGFP mutations result in inCreased brightness (D180Y and V93I), thermal stability (V163A and S175G) and GFP dimerization prevention (A206K).
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J Noncarrier
show more
close
|
| Breeding Considerations |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6J inbred mice. Alternatively, homozygous mice may be bred together.
show more
close
|
| Breeding Strategy |
Hemizygote x Hemizygote
show more
close
|
For Research Use Only.
Related Products