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B6;C3-Tg(CAG-DsRed, -EGFP)5Gae/J

Cat. No.: CEMM-07250121

Common Name: IRG
These IRG transgenic mice are a double-fluorescent, Cre-reporter strain; with widespread expression of red fluorescence prior to Cre recombinase exposure, and green fluorescence following cre-mediated recombination in a pattern determined by cre expression, and should provide a versatile tool for analyzing complex cellular relationships in a wide variety of tissues.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Tg(CAG-DsRed,-EGFP)5Gae
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Allele Name
transgene insertion 5
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Allele Attributes
Reporter
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Gene Symbol
Tg(CAG-DsRed,-EGFP)5Gae
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Gene Name
transgene insertion 5
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Chromosome
UN
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Expressed Genes
GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral
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MGI Accession ID
3809927
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Site of Expression
Widespread expression of red fluorescent protein is observed; following Cre-mediated recombination, GFP is expressed.
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Strain of Origin
(C57BL/6 x C3H)F1
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Molecular Note
The CCAG-R/G expression vector was designed with (from 5' to 3') a Human cytomegalovirus immediate early enhancer and chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP site, a DsRed-Express protein sequence followed by three SV40 polyadenylation signals (DsRed-Express (Clontech) is a red fluorescent protein tetramer with improved solubility and fluorescence detection), a second loxP site, an enhanced green fluorescent protein (EGFP) sequence itself followed by a rabbit beta-globin polyadenylation signal. To minimize integration site effects and possible promoter interference, the CCAG-R/G expression vector was flanked on both sides with two paired copies of anti-sense oriented 5'-HS4 insulator sequences from the chicken beta-globin gene; generating the final "insulator/red/green" targeting vector (pIRG). Line 5 was established and has an estimated transgene copy number of 7. Embryonic and adult tissues exhibit widespread red fluorescent protein expression in the absence of Cre recombinase.
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HUSBANDRY
Suggested Controls
B6C3F1/J
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Breeding Considerations
When maintaining a live colony, hemizygous mice may be bred with non-carrier (wildtype) mice.
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For Research Use Only.
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