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B6.129-Gt(ROSA)26Sortm1Joe/J

Cat. No.: CEMM-07250122

Common Name: ROSA26-GNZ KI
Homozygous ROSA26 GNZ knock-in mice have widespread expression of a nuclear-localized green fluorescent protein/beta-galactosidase fusion protein (GFP-NLS-lacZ or GNZ) once an upstream loxP-flanked STOP sequence is removed. When bred to cre expressing mice, the resulting GNZ fusion protein expression in the offspring allows for enhanced (single cell level) visualization.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm1Joe
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Allele Name
targeted mutation 1
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Allele Attributes
Reporter
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Gene Symbol
Gt(ROSA)26Sor
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Gene Name
gene trap ROSA 26
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Chromosome
6
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Expressed Genes
GFP, Green Fluorescent Protein
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MGI Accession ID
3801312
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Site of Expression
Mice express a nuclear-localized GFP/beta-galactosidase fusion protein (GFP-NLS-LacZ or GNZ) once an upstream loxP-flanked STOP sequence is removed via a tissue-specific Cre recombinase.
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Strain of Origin
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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Molecular Note
A targeting vector was designed with a loxP-flanked STOP cassette (PGK-Neo-polyA followed by transcriptional stop sequence) just upstream of a green fluorescent protein/beta-galactosidase fusion protein sequence with SV40 nuclear localization signal (GFP-NLS-lacZ or GNZ). This entire construct was inserted into the Gt(ROSA)26Sor locus via electroporation.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
Mutant mice were bred to C57BL/6J mice for many generations to establish this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
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Breeding Strategy
Homozygote x Homozygote
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For Research Use Only.
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