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STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
Cat. No.: CEMM-07250046
Common Name: ROSA26eGFP-DTA
ROSA26-eGFP-DTA mice have a loxP-flanked STOP cassette preventing expression of diphtheria toxin fragment A (DTA). Exposure to Cre recombinase removes the lox-stop fragment - resulting in ablation of the cre-expressing cells. These ROSA26-eGFP-DTA mice are useful for Cre-inducible deletion of specific groups of cells.C57BL/6J-congenic ROSA26-eGFP-DTA mice are available as.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(DTA)Jpmb
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein; Dta, Diphtheria toxin A chain
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| MGI Accession ID | show more close |
| Site of Expression |
Expression of EGFP is widespread. When bred to Cre recombinase expressing mice, the loxP-flanked EGFP and stop sequence are removed, and DTA expression is activated, resulting in the specific ablation of Cre-expressing cells.
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| Strain of Origin |
129S/SvEv-Gpi1c
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| Molecular Note |
A targeting vector contained a conditionally expressed DTA construct downstream of a floxed DNA region containing EGFP, a neo cassette and a triple SV40. An EGFP is upstream of the DTA and is constitutively expressed under the control of the ROSA26 protmoter.
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| HUSBANDRY | |
|---|---|
| Breeding Considerations |
When maintaining a live colony, homozygous females are bred with heterozygous males. The donating investigator breeds homozygous females with heterozygous males and reports that some homozygous males may be subfertile, although breeding homozygous mice together has been successful in the past.
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| Breeding Strategy |
Homozygote x Heterozygote
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For Research Use Only.
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