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C57BL/6-Gt(ROSA)26Sortm3(CAG-sfGFP*)Xwy/J
Cat. No.: CEMM-07251018
Common Name: MORF3
MORF3 is one of a set of a Cre reporter tool strains (also including MORF1 and TIGRE-MORF) that use mononucleotide repeat frameshift (MORF) as a translation switch for cell labeling in vivo. MORF3 mice express a cre-dependent tandem "spaghetti monster" fluorescent protein with 20 V5 epitopes (smFP-V5) that is proceeded by a polycytosine repeat (C22) MORF switch under the control of a CAG promoter. Cre recombination combined with a spontaneous frameshift results in sparse and stochastic labeling of neural cells. MORF3 is a multivalent immunoreporter that can be used for sparse labeling of Cre+ neuronal and glial cells.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm3(CAG-sfGFP*)Xwy
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| Allele Name |
targeted mutation 3
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Epitope tag
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Site of Expression |
The CAG promoter allows a widespread pattern of expression, however the _x000D_
Cre-dependent sfGFP reporter contains a farnesylation sequence _x000D_
that targets the reporter to membranous structures.
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| Strain of Origin |
C57BL/6N
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| Molecular Note |
The targeting vector is designed by using the Ai9 plasmid and replacing the tdTomato reporter with a Kozak consensus start site (for strong initiation of translation), two Myc-tags, an optimized C)22(-repeat MORF switch, and a farnesylated tamdem superfolder GFP fluorescent reporter with several V5 epitopes (tdsmFP-V5). Superfolder (sfGFP) is a hyperstable GFP variant that accepts large insertions into its loops while retaining folding and fluorescence. To Create tdsmFP-V5, sfGFP was altered to embed several V5 epitopes (V5 is a 14 amino acid simian virus 5 epitope) in the GFP scaffold - 3 epitopes at the N terminus, 4 epitopes into the internal 172-173 loop and 3 epitopes at the C terminus. The tandem dimer of smFP-V5 contains a total of 20 V5 epitope tags. The inclusion of the V5 epitopes Creates a highly antigenic spaghetti monster fluorescent protein (smGFP) that contains numerous copies of peptide epitopes and simultaneously binds IgG antibodies at each location. The farnesylation sequence (CAAX domain from Ras) targets the reporter to membranous structures including neuronal dendrites and axons. The final vector contains from 5' to 3' a CAG promoter, an FRT site, a loxP-flanked STOP cassette, the tdsmFP-V5 construct (see above), followed by a WPRE, polyA, attB-PGK-FRT neo-pA-attP inserted in to the Gt(ROSA)26Sor locus.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, these mice are bred by homozygote sibling matings.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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