B6;129S4-Dlx1^{tm1(cre/ERT2)Zjh}/J

Dlx1^{tm1(cre/ERT2)Zjh}

transgene insertion 1

Recombinase-expressing; Inducible

Dlx1

distal-less homeobox 1

2

cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene

5295937

Following Tamoxifen administration, Cre recombinase activity is observed in Dlx1 positive cortical GABAergic neurons, striatal neurons, and olfactory neurons.

(C57BL/6 x 129S4/SvJae)F1

A targeting vector was designed to insert a CreERT2 fusion gene, an SV40 polyA signal, and an FRT flanked neo cassette into the initiation codon of the distal-less homeobox 1 locus (Dlx1). The construct was electroporated into (C57BL/6 x 129S4Sv/Jae)-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 mice to originate the colony. The mutation is predicted to result in a null allele. Tamoxifen-inducible Cre recombinase activity recapitulates the endogenous Dlx1 expression pattern, Following tamoxifen administration, Cre recombinase activity is observed in Dlx1 positive cortical GABAergic neurons, striatal neurons, and olfactory neurons.

B6129SF1/J B6129SF2/J

When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J mice. While the donating investigator has not characterized the phenotype of homozygous Dlx1-CreER mice to date, they may be expected to have the same phenotype as other null mutations of this gene (abnormal craniofacial development, cleft palate, and premature death).