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B6.Cg-Cdh6tm1.1(cre/ERT2)Jrs/J
Cat. No.: CEMM-07250731
Common Name: Cdh6CreER (Cdh6-CreER)
The Cdh6CreER (Cdh6-CreER) knock-in/knock-out allele was designed to both abolish endogenous gene function and allow the Cdh6 promoter/enhancer regions to direct tamoxifen-inducible Cre recombinase expression to ON-OFF direction-selective retinal ganglion cells (ooDSGCs) that respond to vertical motion (dorsal and ventral), as well as to starburst amacrine cells. These mice may be useful for tamoxifen-inducible recombination of lox-flanked (floxed) alleles in studying photoreceptors and retinal circuitry.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Cdh6tm1.1(cre/ERT2)Jrs
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| Allele Name |
targeted mutation 1.1
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| Allele Attributes |
Recombinase-expressing; Inducible; Null/Knockout
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| Gene Symbol |
Cdh6
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| Gene Name |
cadherin 6
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| Chromosome |
15
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| Expressed Genes |
cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene
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| MGI Accession ID | show more close |
| Site of Expression |
When induced, Cre recombinase activity recapitulates the endogenous Cdh6 expression pattern in starburst amacrine cells and selective expression in bi-stratified dendrite-retinal ganglion cells.
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| Strain of Origin |
(C57BL/6 x 129S4/SvJae)F1
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| Molecular Note |
The targeting vector contained (from 5' to 3') a cDNA sequence encoding the CreER fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain) with N-terminal 6xMyc tag, followed by an FRT flanked SV40-neo selection cassette. This was inserted into the translational start site in the first coding exon of the gene. Flp-mediated recombination removed the FRT flanked neo cassette. Mice were crossed to reporter animals for analysis.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
The donating investigator reports that homozygous and heterozygous mice are viable and fertile with no reported phenotype abnormalities. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. Alternatively, homozygous mice may be bred together.
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For Research Use Only.
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