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STOCK Gt(ROSA)26Sortm7(CAG-dTomato, -EGFP)Zjh/J
Cat. No.: CEMM-07251074
Common Name: IS2
These IS2 reporter mice harbor a targeted mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette and an FRT-flanked dTomato (dimer Tomato) sequence preventing transcription of a CAG promoter-driven enhanced green fluorescent protein (EGFP). dTomato is expressed following cre-mediated recombination. EGFP is only expressed following cre-mediated and Flp-mediated recombination. This strain may be useful as an intersectional reporter for cre and FLP expression, or as a subtraction reporter for cre expression alone.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm7(CAG-dTomato,-EGFP)Zjh
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| Allele Name |
targeted mutation 7
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
(C57BL/6 x 129S4/SvJae)F1
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| Molecular Note |
The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, an FRT flanked dTomato (dimer Tomato) sequence (dTomato is a DsRed variant that is reported to be a somewhat slow-maturing dimer with low acid sensitivity)), an EGFP, and a Neo-polyA cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6129SF2/J
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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