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FVB(Cg)-Tg(Dhh-cre)1Mejr/J
Cat. No.: CEMM-07250252
The Dhh-cre transgene harbors a nuclear-localized Cre recombinase gene under control of the mouse desert hedgehog (Dhh) promoter/regulatory regions. These transgenic mice may be useful in generating cre-inducible mutations in Sertoli cell precursors (testes development/male germline), endothelial cells, and Schwann cells of the peripheral nervous system.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(Dhh-cre)1Mejr
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| Allele Name |
transgene insertion 1
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Tg(Dhh-cre)1Mejr
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| Gene Name |
transgene insertion 1
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| Chromosome |
UN
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
When bred with mice containing loxP-flanked sequences, Cre recombinase activity is prominently observed in Schwann cell precursors of the developing peripheral nerves (but not in neurons) from embryonic day (E)12. Also at E12, Cre activity is reported in the skin of the snout and parts of the vasculature (blood vessel wall endothelial cells). In E18 testes (before developmental activation of Gata1 expression), Cre recombinase activity is observed pancellularly in pre-Sertoli cells in the testis cords, as well as in endothelial cells of blood vessels. Strong Cre recombinase activity is reported in Dhh-expressing Sertoli cells in the seminiferous tubules of the testis at postnatal day 15 (just before the onset of spermatogenesis When Gata1 is abundantly expressed).
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| Strain of Origin |
FVB/N
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| Molecular Note |
The Cre coding sequence with a nuclear localization peptide sequence was inserted into exon 1 at the start codon in a Dhh genomic clone. The 19 kb construct was microinjected into fertilized FVB/N oocytes.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
FVB/NJ Noncarrier
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| Breeding Considerations |
When maintaining a live colony, hemizygous mice may be bred with wildtype (non-carrier) mice from the colony or with FVB/NJ inbred mice. The donating investigator reports homozygous mice are viable and fertile.
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For Research Use Only.
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