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B6;129-Vamp2tm1(cre/ERT)Nat/J
Cat. No.: CEMM-07250115
This creER knock-in line can be used to produce Vamp2 (vesicle-associated membrane protein 2) gene recombination in many diverse types of neurons following 4-hydroxytamoxifen treatment. Heterozygotes are normal in size and viability. Homozygotes of both genders have reduced viability.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Vamp2tm1(cre/ERT)Nat
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Recombinase-expressing; Inducible
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| Gene Symbol |
Vamp2
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| Gene Name |
vesicle-associated membrane protein 2
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| Chromosome |
11
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| Expressed Genes |
cre/ER, Cre inducible estrogen receptor, bacteriophage P1/Human
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| MGI Accession ID | show more close |
| Site of Expression |
Many diverse neurons (after induction)
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| Strain of Origin |
129
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| Molecular Note |
An IRES-CreER cassette, a tetracycline resistance gene and a Frt-flanked neomycin selection marker were all knocked in to the 3' UTR of the gene. The neomycin selection marker was removed by crossing to a C57BL/6 background Flp recombinase strain. Flp was then bred out of the line. When crossed with a Tg(CAG-Bgeo/ALPP)1Lbe reporter strain and given tamoxifen, Cre expression was detected in a wide variety of neural and glial cells of the brain.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J Wild-type from the colony
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| Breeding Considerations |
When maintained as a live colony, heterozygotes may be bred. Homozygous males and females have poor viability.
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For Research Use Only.
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