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B6;129S4-Gt(ROSA)26Sortm4(CAG-mOrange2, -EGFP, -mKate2)Zjh/J
Cat. No.: CEMM-07250679
Common Name: RGBow reporter
These RGBow reporter mice express one of three membrane-tethered fluorescent proteins in a random manner upon cre-mediated recombination; this allows labeling of individual cell types with distinguishable color variations in cre recombined cells.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm4(CAG-mOrange2,-EGFP,-mKate2)Zjh
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| Allele Name |
targeted mutation 4
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral
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| MGI Accession ID | show more close |
| Site of Expression |
One of either mOrange2 (monomeric RFP), EGFP, or mKate2 (monomeric far-red RFP) is expressed in Cre-expressing cells after Cre recombination.
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| Strain of Origin |
(C57BL/6 x 129S4/SvJae)F1
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| Molecular Note |
The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), and three farnesylated fluorescent protein sequences uniquely flanked with pairs of incompatible lox sites. Specifically, the coding region contains (from 5' to 3') a loxP site, a lox2272 site, a loxN site, a transcriptional STOP signal, mOrange2 sequence (derived from a monomeric red fluorescent protein) with polyA sequence, a loxP site, EGFP (enhanced green fluorescent protein) with polyA sequence, a lox2272 site, mKate2 (derived from a monomeric far-red fluorescent protein). This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
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| HUSBANDRY | |
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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