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B6.129(Cg)-Phox2btm1Rth/J
Cat. No.: CEMM-07250564
In this targeted mutant strain, exon 3 of the mouse Phox2b gene is flanked by loxP sites and exon 3 of the human PHOX2B gene, modified with an 8 bp deletion and EGFP marker, has been placed downstream. Cre-mediated excision of the floxed exon knocks out expression of the mouse form of protein, but allows the mutant human form and EGFP to be expressed.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Phox2btm1Rth
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Humanized sequence
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| Gene Symbol |
Phox2b
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| Gene Name |
paired-like homeobox 2b
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| Chromosome |
5
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| Expressed Genes |
GFP, Green Fluorescent Protein; PHOX2B, paired like homeobox 2B, human
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| MGI Accession ID | show more close |
| Site of Expression |
EGFP reporter is expressed in specific hindbrain nuclei and enteric neurons.
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| Strain of Origin |
129
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| Molecular Note |
Exon 3 of the mouse Phox2b gene was flanked by loxP sites. An FRT flanked neomycin cassette, human exon 3 (modified with an 8 bp deletion, GGCCCGGG) and associated with an EGFP reporter, was inserted downstream. Flp-mediated recombination removed the selection cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
Homozygous and heterozygous mice are viable and fertile.
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For Research Use Only.
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