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B6.Cg-Rxfp1em1(cre)Ngai/TasicJ

Cat. No.: CEMM-07251104

Common Name: Rxfp1-P2A-Cre
Rxfp1-P2A-Cre knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous relaxin/insulin-like family peptide receptor 1 promoter/enhancer sequences. These mice may be used to generate conditional mutations for studying function of Rxfp1-expressing cells.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Rxfp1em1(cre)Ngai
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Allele Name
endonuclease-mediated mutation 1
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Allele Attributes
Recombinase-expressing
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Gene Symbol
Rxfp1
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Gene Name
relaxin/insulin-like family peptide receptor 1
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Chromosome
3
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MGI Accession ID
6502651
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Strain of Origin
(C57BL/6 x 129S4/SvJae)F1
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Molecular Note
The bicistronic Rxfp1-P2A-Cre knock-in allele was Created using homologous recombination (aided by targeted CRISPR/Cas9 endonuclease activity) to insert a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping) and a Cre recombinase gene (Cre) the gene. The targeting vector, Cas9 endonuclease, and guide-RNA were mixed and injected into the v6.5 mouse embryonic stem cells. Colonies grown from transfected cells were directly sCreened for successful integration and were aggregated with albino morula and implantedinto pseudopregnant females, which produced germ-line-competent chimeric founders.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. it has not been attempted to make this strain homozygous.
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Breeding Strategy
Heterozygote x Heterozygote
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For Research Use Only.
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