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B6.129S4-Gt(ROSA)26Sortm1(CAG-EGFP/Rpl10a, -birA)Wtp/J
Cat. No.: CEMM-07250473
Common Name: Rosa26fsTRAP
fs-TRAP mice are designed to express the EGFP-L10a ribosome tag following exposure to Cre recombinase, and then express 2xHA-tagged BirA (biotin ligase) following exposure to FLP recombinase. This is a tool for the isolation and purification of ribosome-bound proteins and protein complexes from tissues.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-EGFP/Rpl10a,-birA)Wtp
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein; birA, biotin ligase), bacteria
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| MGI Accession ID | show more close |
| Site of Expression |
The EGFP-L10a fusion protein will be expressed in tissues where promoters driving Cre recombinase are expressed.
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| Strain of Origin |
129S4/SvJae
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| Molecular Note |
The fs-TRAP targeting vector was designed with, from 5' to 3', a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a floxed-neomycin (neo) resistance cassette and polyadenylation (polyA) sequence, a FRT flanked enhanced green fluorescence protein (EGFP)-L10a (ribosomal protein) fusion protein, a 2xHA (hemagglutinin) tagged bacterial birA, bifunctional protein (biotin ligase), gene, and a polyA sequence. This targeting vector was inserted into the Gt(ROSA)26Sor gene.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, these mice can be bred as homozygotes.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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