B6(129S4)-Et(icre/ERT2)14208Rdav/J

Cat. No.: CEMM-07250238

These mice express the tamoxifen-inducible iCre-ERT2 fusion protein (iCre-ERT2) under the control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. These mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in brain tissues.

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Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Et(icre/ERT2)14208Rdav show more close
Allele Name	enhancer trap 14208 show more close
Allele Attributes	Recombinase-expressing; Inducible show more close
Gene Symbol	Et(icre/ERT2)14208Rdav show more close
Gene Name	enhancer trap 14208 show more close
Chromosome	UN show more close
Expressed Genes	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene show more close
MGI Accession ID	4947569 show more close
Site of Expression	Cre recombinase activity is observed in the following brain tissues: scattered cells in the neocortex, hippocampus, amygdala, striatum, thalamus, hypothalamus, midbrain, pons, medulla, and many cerebellar granule cells. show more close
Strain of Origin	C57BL/6J show more close
Molecular Note	An enhancer trap lentiviral transgenic approach was used to generate these mice. The transgene was designed with a self-inactivating lentiviral vector backbone (FUGW), a human synapsin minimal promoter (-244 to +47), a beta-globin intron, an iCre /ERT2 fusion (iCre; improved with mammalian codon usage, no putative cryptic splice sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals), fused to a mutated human estrogen receptorligand binding domain (ERT2), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability. This packaged lentivirus was injected subzonally (under the zona pellucida) into 1-2 cell stage C57BL/6J mouse embryos. Founders were crossed to C57BL/6 animals. Animals were crossed to B6-Gt(ROSA)26Sortm1Sor mice to assess Cre activity/transmission. Cre positive, lacZ negative mice were subsequently bred to C57BL/6 mice to Create this line. show more close

HUSBANDRY
Suggested Controls	C57BL/6J show more close
Breeding Considerations	When maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (non-carrier) siblings, or to C57BL/6J inbred mice. show more close

For Research Use Only.