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B6;129S-Gt(ROSA)26Sortm2.1Ksvo/J
Cat. No.: CEMM-07250549
Common Name: R26 LSL FSF ReaChR-mCitrine
ReaChR protein (a red-shifted channelrhodopsin) was tagged with mCitrine fluorescent protein and placed under the conditional control of the Gt(ROSA)26Sor promoter. Excision of two stop cassettes (one flanked by loxP sites, another flanked by FRT sites) enables fluorescent expression. As an optogenetic tool, this strain is useful for the selective activation and mapping of neuron populations.Anecdotal reports suggest that the FRT-flanked Stop cassette may* be showing FRT-site read through in this line, effectively making this a cre-dependent strain only.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm2.1Ksvo
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| Allele Name |
targeted mutation 2.1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
YFP, Yellow Fluorescent Protein, jellyfish
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| MGI Accession ID | show more close |
| Site of Expression |
The fluorescent protein mCitrine will be expressed in cells/tissues where promoters driving Cre recombinase and Flp recombinase are expressed.
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| Strain of Origin |
(C57BL/6J x 129S6/SvEvTac)F1
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| Molecular Note |
A CAG promoter (cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promoter), FRT flanked stop cassette, loxP-flanked stop cassette, ReaChR red-shifted channelrhodopsin, fluorescent reporter mCitrine, woodchuck hepatitis post-transcriptional regulatory element (WPRE), poly A (pA) tail, and AttB/AttP-flanked neomycin resistance cassette were introduced to intron 1 of the Gt(ROSA)26Sor gene. PhiC31o-mediated recombination removed the neo cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6129SF2/J
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| Breeding Considerations |
Homozygotes and heterozygotes are viable and fertile.
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| Breeding Strategy |
Homozygote x Homozygote
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| Coat Color |
Coat colors observed are Agouti and Black.
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For Research Use Only.
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