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STOCK Igs3tm2.1(ACTB-EGFP*, -tdTomato)Luo/J
Cat. No.: CEMM-07250381
Common Name: Miya10GT (M10GT)
The Miya10GT (M10GT) transgene has the CMV enhancer/chicken beta-actin core promoter upstream of a frt-flanked "MADM GT" cassette, all inserted into an intergenic region on chromosome 10 (~21 Mbp; between the Ahi1 and Myb loci). These mutant mice are designed for MADM-10 (mosaic analysis with double markers on chromosome 10), and provide a tool to generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows Cre recombinase-induced fluorescent labeling of daughter cells to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation and mitosis.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs3tm2.1(ACTB-EGFP*,-tdTomato)Luo
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| Allele Name |
targeted mutation 2.1
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| Allele Attributes |
Reporter; Null/Knockout
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| Gene Symbol |
Igs3
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| Gene Name |
intergenic site 3
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| Chromosome |
10
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| Expressed Genes |
GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral
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| MGI Accession ID | show more close |
| Site of Expression |
Cre recombination results in tdTomato and EGFP mosaic expression in Cre-expressing tissues When using the MADM-10 system.
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| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
The pMADMalpha transgene was designed with the CMV enhancer/chicken beta-actin core promoter (pCA) promoter, a FRT site, the MADM GT cassette, and second FRT site, the MADM TG cassette, a third FRT site, and an SV40 T-antigen poly(A) signal. The MADM GT cassette used here has the N-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP first exon; nucleotides 1-274), a beta-globin intronic sequence (containing a loxP-flanked neomycin resistance gene), and ATG-less tdTomato sequence (nucleotides 3-1548 including stop codon) tagged with three copies of the Myc epitope at its C-terminus (tdT3MycATG-less). The MADM TG cassette used here has an ATG start codon, a beta-globin intronic sequence (containing a loxP-flanked neomycin resistance gene), and the C-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP second exon; nucleotides 275-735 including stop codon). The transgene was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells were sCreened for intact single-copy transgenes with integration in intergenic regions close to any centromere. ES cells were identified with an intact single-copy of the transgene inserted into an intergenic region at ~21 Mbp on chromosome 10 between the Ahi1 and Myb loci; this new insertion locus was named Miya10 (or M10). The transgene inserted into an intergenic region at ~21 Mbp on chromosome 10 between the Ahi1 and Myb loci; this new insertion locus was named Miya10 (or M10). Cre-mediated recombination removed the neo cassette.
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| HUSBANDRY | |
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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