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STOCK Igs2tm1(ACTB-EGFP, -tdTomato)Zng/Igs2tm2(ACTB-tdTomato, -EGFP)Zng/J

Cat. No.: CEMM-07250817

Common Name: GT11ML/TG11ML (MADM-ML-11GT/TG) trans-heterozygous
GT11ML/TG11ML (MADM-ML-11GT/TG compound heterozygous mice harbor reciprocal mutations on homologous chromosomes ("trans-heterozygous"), and are designed for MADM (mosaic analysis with double markers). Exposure to Cre- or FLP-recombinase induces fluorescent protein expression. Compared to the single loxP approach of the original MADM designs, the multiple self-recognizing lox variant sites present in the MADM-ML system result in significantly improved recombination efficiency (4-8 fold higher) with no negative impact on G2-X segregation percentage (G2-X segregation produces the desired fluorescent homozygous mutant daughter cells). This MADM-ML system allows Cre recombinase-induced fluorescent labeling of daughter cells to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation, mitosis, and imprinting.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Igs2tm2(ACTB-tdTomato,-EGFP)Zng
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Allele Name
targeted mutation 2
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Allele Attributes
Reporter; Null/Knockout
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Gene Symbol
Igs2
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Gene Name
intergenic site 2
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Chromosome
11
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Expressed Genes
GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral
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MGI Accession ID
5520975
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Site of Expression
EGFP and tdTomato will be expressed in cells/tissues where promoters driving Cre recombinase and Flp recombinase are expressed.
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Strain of Origin
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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Molecular Note
The "multiple lox" MADM-ML-11 targeting vector has a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the "TG MADM-ML" cassette. The "TG MADM-ML" cassette is designed with the N-terminal portion of a red fluorescent protein (tdTomato; nucleotide 1-3),a beta-globin intronic sequence (containing an FRT lox5171-lox2272-FRT loxP-Neo-loxP),the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; nucleotide 274-724), and an SV40 T-antigen poly(A) signal.
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For Research Use Only.
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