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B6.129P2(Cg)-Gt(ROSA)26Sortm1(tTA)Roos/J
Cat. No.: CEMM-07250209
Common Name: ROSA:LNL:tTA
These ROSA:LNL:tTA mice can be used to generate compound mutant mice in which the tissue specificity of a cre-transgenic line and the tetracycline or dox responsiveness of the tTA/TRE-controlled transgenes can be combined to regulate expression of the target gene.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(tTA)Roos
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Transactivator
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
tTA, tetracycline-controlled transactivator, E. coli
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| MGI Accession ID | show more close |
| Site of Expression |
Widespread expression of the tetracycline-controlled transactivator (tTA) protein is initiated after removal of an upstream loxP-flanked STOP cassette by Cre recombinase. Thus the expression pattern of tTA is defined by the expression pattern of Cre recombinase.
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| Strain of Origin |
129P2/OlaHsd-Hprtb-m3
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| Molecular Note |
A targeting vector was designed to insert a loxP-flanked STOP cassette (containing a neomycin resistance cassette and transcriptional STOP element) just upstream of a modified/optimized form of tetracycline controlled transactivator ("mtTA") into the Gt(ROSA)26Sor locus. and chimeric mice were established.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Mutant mice were bred with C57BL/6J inbred mice using a marker-assisted, speed congenic approach to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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