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B6.Cg-Igs7tm162.1(tetO-GCaMP6s, CAG-tTA2)Hze/J
Cat. No.: CEMM-07250875
Common Name: Ai162(TIT2L-GC6s-ICL-tTA2)-D (or Ai162D)
Ai162(TIT2L-GC6s-ICL-tTA2)-D (also called Ai162D) mice are a Cre-dependent, Tet-controllable, fluorescent calcium-indicator tool strain - created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). Exposure to Cre recombinase removes both STOP cassettes, resulting in expression of tTA2 and GCaMP6s (an ultrasensitive detector of single neuronal action potentials with slower decay and response kinetics). GCaMP6s expression may then be diminished by doxycycline.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs7tm162.1(tetO-GCaMP6s,CAG-tTA2)Hze
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| Allele Name |
targeted mutation 162.1
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| Allele Attributes |
Reporter; Inducible
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| Gene Symbol |
Igs7
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| Gene Name |
intergenic site 7
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| Chromosome |
9
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| Expressed Genes |
tTA, tetracycline-controlled transactivator, E. coli; GCaMP, Genetically encoded calcium indicator
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| MGI Accession ID | show more close |
| Site of Expression |
When bred to mice expressing Cre recombinase, the offspring will have both floxed-STOP cassettes deleted in the Cre-expressing cells/tissues - resulting in GCaMP6s expression and tTA2 expression.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The vector is designed with (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a Tet response element/promoter (TRE2; details below), a loxP-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), a GCaMP6 slow variant calcium indicator sequence (GCaMP6s), a WPRE (to enhance the mRNA transcript stability), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from pCAGGS), a lox2272-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-TKpA), a synthetic modified tetracycline-regulated transactivator gene (tTA2S), a WPRE, a BGH polyA, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site. The TRE2 promoter used here is Tet-responsive P>hCMV*-1min CMVhCMV*-1< is silent in the absence of tTA or rtTA binding to tetO. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence and replaced it with the recombined AttB/AttP site (AttL).
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For Research Use Only.
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