B6.129P2-Myd88^tm1Hlz/J

Myd88^tm1Hlz

targeted mutation 1

Conditional ready (e.g. floxed); Null/Knockout

Myd88

myeloid differentiation primary response gene 88

9

5442766

129P2/OlaHsd

An intron gene trap cassette, containing a loxP site, Engailed 2 splice acceptor/STOP (En-2/stp), IRES, lacZ, downstream element (DSE) and floxed neo cassette, was targeted to intron 1. This allele is both a knock-out and conditional-ready allele, as it can be reverted back to wild-type expression through Cre-mediated recombination. Western blot analysis confirmed the absence of protein expression in bone marrow derived macrophage stimulated with (S)-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-N-palmitoyl-(R)-Cys-(S)-Ser-(S)-Lys4-OH (P3C).

C57BL/6NJ

When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6NJ inbred mice. Alternatively, homozygous mice may be bred together (see below).When maintained at high health status (specific pathogen-free), the donating investigator reports that homozygous (MyD88LSL/LSL) mice are viable and fertile with normal lifespan, and may be bred together. Importantly, global MYD88-deficiency is associated with increased susceptibility to bacterial and viral infections, a number of immune system abnormalities, as well as hematopoietic system, molecular signaling and apoptotic abnormalities. As such, and similar to other immunodeficient strains, maintenance in high health status (specific pathogen-free) vivaria promotes overall colony health. If MYD88-deficient animals are maintained in low health barrier rooms, the use of medicated water (e.g. sulfatrim/trimethoprim-sulfa or enrofloxacin/Baytril) may be warranted to increase overall colony health.

Homozygote x Homozygote