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B6.Cg-Gt(ROSA)26Sortm1.2(CAG-tdTomato, -EGFP)Pjen/J
Cat. No.: CEMM-07250620
Common Name: RC::RLTG
RC::RLTG dual-recombinase responsive indicator is designed with a rox-flanked STOP and loxP-flanked tdTomato::STOP all preventing transcription of eGFP. Dre recombinase results in high tdTomato fluorescence, and further exposure to Cre recombinase results in robust eGFP fluorescence. The RC::RLTG allele and its derivatives are useful for fluorescent labeling of cells/tissues in embryonic or adult mice. These mice allow intersectional genetic fate mapping of different cell subpopulations defined by the overlap of two gene expression domains.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1.2(CAG-tdTomato,-EGFP)Pjen
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| Allele Name |
targeted mutation 1.2
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
RFP, Red Fluorescent Protein, coral; GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
EGFP and tdTomato fluorescent protein will be expressed in cells where promoters driving Flp recombinase are expressed.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The targeting construct contains a rox-flanked STOP, FRT flanked STOP and loxP-flanked tdTomato::STOP upstream of the eGFP fluorescent protein, inserted into the Gt(ROSA)26Sor locus. Flp-mediated recombination removed the FRT flanked STOP.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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