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B6;129S6-Gt(ROSA)26Sortm10(CAG-Syp/EGFP*, -tdTomato)Dym/J
Cat. No.: CEMM-07250783
Common Name: RC::FPSit
The RC::FPSit dual-recombinase intersectional allele has a frt-flanked STOP and loxP-flanked STOP that prevent transcription of synaptophysin-EGFP fusion protein and tdTomato. Exposure to FLP and Cre recombinase results in strong EGFP expression enriched at the synapse/synaptic vesicle (axonal boutons [terminal and en passant]) of the neuron subtype(s) defined by the overlap of FLP/Cre expression.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm10(CAG-Syp/EGFP*,-tdTomato)Dym
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| Allele Name |
targeted mutation 10
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; No functional change
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral
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| MGI Accession ID | show more close |
| Site of Expression |
After removal of both STOP cassettes by FLP- and Cre-recombination, fluorescent protein expression is observed in those cells where FLP and Cre expression overlaps - strong EGFP expression is enriched at the synapse/synaptic vesicle (axonal boutons [terminal and en passant]), whereas weak tdTomato expression is observed. EGFP expression is also observed in the soma and neurites to a certain degree.
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| Strain of Origin |
129S6/SvEvTac
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| Molecular Note |
The targeting construct includes from 5' to 3' a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, an FRT flanked STOP cassette, a loxP-flanked STOP cassette, a synaptophysin-GFP fusion gene (described below), IRES, a tdTomato gene, a WPRE, and a rabbit beta-globin polyA sequence. The synaptophysin-GFP fusion protein is a full-length mouse synaptophysin gene fused to a mutant enhanced green fluorescent protein (mut4EGFP; harboring the V163A/S175G codon changes for inCreased ability to fold properly at 37 degrees C).
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For Research Use Only.
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