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STOCK Tg(Pmch-cre)1Lowl/J
Cat. No.: CEMM-07250286
Common Name: Mch-Cre
These transgenic mice have the murine pro-melanin-concentrating hormone (Pmch or MCH) promoter/enhancer regions within the BAC transgene directing expression of Cre-recombinase. These mice may be useful for studying MCH-neuronal activities in controlling energy balance, glucose homeostasis, sleep, locomotion, and reward-related behaviors.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(Pmch-cre)1Lowl
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| Allele Name |
transgene insertion 1
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Tg(Pmch-cre)1Lowl
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| Gene Name |
transgene insertion 1
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| Chromosome |
UN
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Cre expression is directed specifically to the melanin-concentrating hormone (MCH) neurons of the hypothalamus and zona incerta.
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| Strain of Origin |
FVB/NJ
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| Molecular Note |
Homologous recombination with EL250 cells was induced to insert a Cre-polyA-FRT Neo-FRT cassette into a BAC containing the Pmch (pro-melanin-concentrating hormone; MCH) at the Pmch translational start site with concomitant replacement of the 26 nucleotides following it. Arabinose-induced Flp recombination in bacterial cells removed the kanamycin cassette. The purified, linearized construct was injected into the pronuclei of FVB one-cell embryos. Eight lines were generated and crossed to Z/EG reporter animals. 2 lines exhibited GFP expression in the lateral hypothalamus and zona incerta, known sites of endogenous Pmch expression. Cre expression in one of these lines of characterized further.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
FVB/NJ
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| Breeding Considerations |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to FVB/NJ inbred mice. The donating investigator reports they have not tried to generate homozygous mice.
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For Research Use Only.
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