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B6(Cg)-Etv1tm1.1(cre/ERT2)Zjh/J

Cat. No.: CEMM-07250253

Common Name: ER81-CreER
The Etv1-CreERT2 knock-in allele was designed to both abolish ets variant gene 1 (Etv1) gene function and direct CreERT2 fusion protein expression to ER81 positive neurons (including Layer5 pyramidal neurons) by the endogenous Etv1 promoter/enhancer elements. Cre-ERT2 fusion gene activity is inducible by tamoxifen administration.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Etv1tm1.1(cre/ERT2)Zjh
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Allele Name
targeted mutation 1.1
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Allele Attributes
Recombinase-expressing; Inducible
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Gene Symbol
Etv1
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Gene Name
ets variant 1
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Chromosome
12
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Expressed Genes
cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene
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MGI Accession ID
4838417
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Site of Expression
Cre recombinase activity is observed in ER81 positive cortical neurons (including Layer5 pyramidal neurons).
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Strain of Origin
C57BL/6
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Molecular Note
A targeting vector was designed to insert a Cre/ERT2 fusion gene, an SV40 polyA signal, and an FRT flanked neo cassette into the initiation codon of the ets variant gene 1 locus (Etv1). This construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with white C57BL/6 mice (harboring the Tyrc-2J mutation) to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J mice. The donating investigator has not fully characterized the homozygous phenotype to date.
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Breeding Strategy
Heterozygote x +/+ sibling
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For Research Use Only.
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