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STOCK En2tm5.1Alj/J

Cat. No.: CEMM-07250088

These En2flox-taulacZ (En2-floxLacZ, En2tau-lacZ, or En2-tau-lacZ) mutant mice harbor a loxP-flanked tau β-galactosidase "knock-in" allele that also abolishes engrailed 2 (En2) gene function, and may be useful for conditional reporter protein expression in En2-expressing tissues (including the early mesencephalon and rhombomere 1, and developing cerebellum and jaw muscles), as well as for conditional restoration of En2 function.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
En2tm5.1Alj
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Allele Name
targeted mutation 5.1
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Allele Attributes
Conditional ready (e.g. floxed); No functional change
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Gene Symbol
En2
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Gene Name
engrailed 2
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Chromosome
5
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Expressed Genes
lacZ, beta-galactosidase, E. coli
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MGI Accession ID
3789089
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Site of Expression
LoxP-flanked tau ?-galactosidase expression mimics and blocks that of the endogenous targeted gene. When bred to Cre recombinase mice, resulting offspring will have the LacZ reporter deleted in the Cre-expressing tissue(s) with subsequent restoration of the targeted gene's translation.
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Strain of Origin
129S6/SvEvTac
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Molecular Note
A targeting vector was designed to insert a loxP-flanked tau-lacZ cassette followed by a neo cassette and then a third loxP site into the 5'-UTR of the targeted gene. Cre mediated recombination then removed the neo cassette leaving the floxed tau-lacZ cassette intact.
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HUSBANDRY
Suggested Controls
Wild-type from the colony
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Breeding Considerations
When maintaining a live colony, heterozygous mice may be bred together or with wildtype siblings. The donating investigator reports that homozygous mice are "semi-fertile" and do not breed well.
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For Research Use Only.
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