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B6;129S6-Gt(ROSA)26Sortm8(CAG-mCherry, -EGFP)Dym/J

Cat. No.: CEMM-07250736

Common Name: RC::FrePe dual-recombinase responsive fluorescent
The RC::FrePe dual-recombinase responsive fluorescent allele has a frt-flanked STOP and loxP-flanked mCherry::STOP all preventing transcription of eGFP. FLP recombinase results in mCherry fluorescence, and further exposure to Cre recombinase results in eGFP fluorescence in the overlapping populations. The RC::FrePe allele and its derivatives are useful for fluorescent labeling of cells/tissues in embryonic or adult mice. These mice allow intersectional genetic fate mapping of different cell subpopulations defined by the overlap of two gene expression domains.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm8(CAG-mCherry,-EGFP)Dym
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Allele Name
targeted mutation 8
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Allele Attributes
Conditional ready (e.g. floxed); No functional change
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Gene Symbol
Gt(ROSA)26Sor
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Gene Name
gene trap ROSA 26
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Chromosome
6
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Expressed Genes
RFP, Red Fluorescent Protein, coral; GFP, Green Fluorescent Protein
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MGI Accession ID
5320888
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Site of Expression
After removal of the first STOP cassettes by FLP recombination, mCherry fluorescence is expected in Flp-expressing serotonergic neurons. Further exposure to Cre recombinase removes the floxed-tdTomato::STOP, resulting in eGFP fluorescence.
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Strain of Origin
Not Specified
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Molecular Note
The CAG promoter drives ubiquitous expression of an FRT disrupted red mCherry and loxP-disrupted EGFP.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
Mice homozygous for the RC::FrePe allele are viable and fertile with no reported gross physical or behavioral abnormalities. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred animals. Alternatively, homozygous mice may be bred together.
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Breeding Strategy
Heterozygote x Heterozygote
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For Research Use Only.
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