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B6;129-Gt(ROSA)26Sortm9(CAG-GFP*)Nat/J
Cat. No.: CEMM-07250583
This reduced recombination, nuclear-localized GFP reporter strain (R26 mLSL-nGFP) enables extremely sparse cre-mediated labeling of cells and is appropriate for applications such as lineage tracing.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm9(CAG-GFP*)Nat
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| Allele Name |
targeted mutation 9
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
Upon induction with 4-hydroxyTamoxifen (4HT) GFP is observed in Cre-expressing tissues.
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| Strain of Origin |
129
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| Molecular Note |
The targeting vector containins (from 5' to 3') a CAG promoter, a mutant loxP63 (ATAACTTCGTATAGCCTACATTATACGAAGTTAT)-triple poly A/stop-wild type loxP (ATAACTTCGTATAGCATACATTATACGAAGTTAT), a nuclear-localized GFP with a C-terminal 6-myc epitope tag, a bovine growth hormone 3' UTR, and a Frt-PGKneo-Frt cassette. The stop cassette consists of three tandem SV40 polyadenylation/transcription termination sequences.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
Heterozygotes and homozygotes are viable and fertile.
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For Research Use Only.
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