B6.Cg-Tg(Gfap-cre)77.6Mvs/J

Tg(Gfap-cre)77.6Mvs

transgene insertion 77.6

Recombinase-expressing

Tg(Gfap-cre)77.6Mvs

transgene insertion 77.6

UN

Cre, Cre recombinase, bacteriophage P1

3838840

Cre recombinase activity is is observed in most astrocytes throughout the healthy brain and spinal cord and to essentially all astrocytes after Central Nervous System (CNS) injury. Cre recombinase activity is also observed in a subpopulation of the adult stems in the subventricular zone. In contrast to Tg(Gfap-cre)73.12Mvs, there is no targeting of postnatal or adult neural stem cells or their progeny in the hippocampus or other brain regions in Tg(Gfap-cre)77.6Mvs.

(BALB/c x C57BL/6NHsd)F1

The bacterial Cre recombinase gene was inserted into the first exon of an expression cassette (clone 445) that contains all the introns, promoter regulatory elements, exons, and 2.5 kb of 5' and 2 kb of 3' flanking DNA of the astroglia-specific mouse glial fibrillary acidic protein gene. GFAP is not expressed from the transgene due to deletion of part of exon 1. Cre expression was detected in a small population of cells within the subependymal zone in the adult brain. No Cre expression or activity was detected during embryonic development or in the hippocampus.

C57BL/6J

When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony.

Noncarrier x Hemizygote