B6.Cg-Gt(ROSA)26Sor^{tm75.1(CAG-tdTomato*)Hze}/J

Gt(ROSA)26Sor^{tm75.1(CAG-tdTomato*)Hze}

targeted mutation 75.1

Conditional ready (e.g. floxed); Reporter; No functional change

Gt(ROSA)26Sor

gene trap ROSA 26

6

RFP, Red Fluorescent Protein, coral

5603432

After exposure to Cre recombinase, expression of the tdTomato fluroescent protein is directed to the nuclei.

(129S6/SvEvTac x C57BL/6NCrl)F1

The Rosa-pCAG-LSL-ntdTomato-WPRE-bGHpA-AttB-PGK-neo-polyA-AttP targeting vector was designed with (from 5' to 3')a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxP-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), the ntdTomato coding sequence (nT; a tdTomato fluorescent protein sequence with amnio-terminal addition of a 30bp fragment encoding a nuclear localization signal), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance mRNA transcript stability), a bovine growth hormone polyA signal, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, and an AttP site. PhiC31o-mediated recombination removed the AttB/AttP-flanked PGK-Neo-polyA cassette and replaced it with the recombined AttB/AttP site (AttL).

C57BL/6J

When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice. The donating investigator has not attempted to generate homozygous mice to date.