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STOCK Igs2tm2(ACTB-tdTomato, -EGFP)Luo/J

Cat. No.: CEMM-07250283

Common Name: HIPP11TG
The MADM-11TG allele with enhanced green fluorescent protein is inserted into the Igs2 (formerly Hipp11) locus on chromosome 11. This MADM system allows Cre- or FLP-mediated fluorescent labeling of daughter cells to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation and mitosis.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Igs2tm2(ACTB-tdTomato,-EGFP)Luo
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Allele Name
targeted mutation 2
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Allele Attributes
Reporter; Null/Knockout
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Gene Symbol
Igs2
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Gene Name
intergenic site 2
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Chromosome
11
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Expressed Genes
GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral
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MGI Accession ID
4837863
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Site of Expression
Mutant daughter cells express GFP, while the sibling wildtype cells are labeled with RFP.
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Strain of Origin
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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Molecular Note
The TG MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a red fluorescent protein (tdTomato; aa 1-3), a beta-globin intronic sequence containing an FRT site and loxP-flanked neomycin resistance gene, the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 274-724), and an SV40 T-antigen poly(A) signal. This entire TG MADM construct was inserted into the "Hipp11" locus on chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci).
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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