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B6;129-Pou4f1tm2.1Nat/J
Cat. No.: CEMM-07250181
These mice possess loxP sites on either side of the Pou4f1 (POU domain, class 4, transcription factor 1) coding region followed by an alkaline phosphatase (AP) reporter. When crossed with a cre recombinase-expressing strain, offspring express AP in the retina, several brain nuclei, dorsal root ganglia, trigeminal ganglion, and other cells/tissues.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Pou4f1tm2.1Nat
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| Allele Name |
targeted mutation 2.1
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| Allele Attributes |
Conditional ready (e.g. floxed); No functional change
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| Gene Symbol |
Pou4f1
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| Gene Name |
POU domain
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| Chromosome |
14
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| MGI Accession ID | show more close |
| Strain of Origin |
129
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| Molecular Note |
The locus was targeted to have its entire coding region floxed and to express a reporter gene after Cre-mediated recombination. A loxP site was inserted in the upstream untranslated region 42 base pairs before the initiator ATG codon. Three repeats of the SV40 early region transcription terminator were added to the untranslated region 48 base pairs downstream of the termination codon, followed by a second loxP site and the coding region of human placental alkaline phosphatase (AP). A FRT flanked neomycin selection cassette followed the AP coding region and was subsequently removed by crossing to mice expressing FLP recombinase. Following Cre-mediated recombination, AP activity is detectable in the retina and brain.
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| HUSBANDRY | |
|---|---|
| Breeding Considerations |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
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For Research Use Only.
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