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C57BL/6N-Il1r1tm1Quan/J
Cat. No.: CEMM-07250524
Common Name: IL-1R1 restore knock-in (IL-1R1r)
The interleukin 1 type I receptor restore allele (IL-1R1r) is a functional null prior to Cre recombinase exposure. These mice allow Cre recombinase-inducible restoration of functional IL-1R1 expression, and visualization of IL-1R1-expressing cells with concordant labeling of IL-1R1 mRNA (by tdTomato fluorescence) and protein (by 3xHA epitope). IL-1R1r mice may be useful for studying cell-type-specific functions of IL-1R1 in neuroimmunity and neuroinflammation.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Il1r1tm1Quan
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Null/Knockout
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| Gene Symbol |
Il1r1
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| Gene Name |
interleukin 1 receptor
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| Chromosome |
1
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| Expressed Genes |
Il1r1, interleukin 1 receptor, type I, mouse, laboratory; HA, influenza hemagglutinin; RFP, Red Fluorescent Protein, coral
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| MGI Accession ID | show more close |
| Site of Expression |
Hemagglutinin epitope tags (HA), tdTomato, and Il1r1 will be expressed in in the cytoplasm of IL-1R1 mRNA-producing cells following Cre recombinase exposure.
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| Strain of Origin |
C57BL/6NTac
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| Molecular Note |
The allele contains two knock-in sequences incorporated by two rounds of homologous recombineering. The floxed target I sequence is inserted between exon IX and exon X; it is composed of a loxP site, a mouse engrailed 2 splice acceptor sequence, a destabilization domain (DD), a 2xPolyA transcriptional STOP sequence, a neo cassette and a second loxP site.Target II sequence is inserted in place of the Il1r1 stop codon in exon XI; it is composed of 3 hemagglutinin tags (3xHA), a STOP codon fluorescent protein, an internal ribosome entry site (IRES), a tdTomato fluorescent protein sequence, a single FRT site and a STOP codon. Mice containing target I do not produce a functional protein.
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For Research Use Only.
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