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STOCK Trpa1tm2Kykw Tg(CAG-cre/Esr1*)5Amc/J
Cat. No.: CEMM-07250131
Common Name: Trpa1 CKO/CKO deltaNeo Tg(cre/Esr1)5Amc/J
This Trpa1CKO;pCAGGCre-ERTM compound mutant has a floxed allele of the Trpa1 (transient receptor potential cation channel, subfamily A, member 1) gene in addition to a tamoxifen inducible cre transgene driven by the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer (see). Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. Tamoxifen administration induces cre recombination within the Trpa1 allele, excising the S5/S6 transmembrane domains. Animals not treated with tamoxifen display no observable phenotype.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Trpa1tm2Kykw
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Conditional ready (e.g. floxed); No functional change
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| Gene Symbol |
Trpa1
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| Gene Name |
transient receptor potential cation channel
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| Chromosome |
1
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| MGI Accession ID | show more close |
| Site of Expression |
Tamoxifen-inducible cre; widespread pattern of expression; Tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice
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| Strain of Origin |
129S6/SvEvTac
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| Molecular Note |
LoxP sites were placed on either side of exons 22-24 (encoding the S5/S6 transmembrane domains) and an FRT flanked neomycin resistance cassette inserted in the opposite transcriptional orientation in intron 24. The neomycin cassette was excised by crossing these mice to a Gt(ROSA)26Sor-driven Flp strain on a 129S4/SvJaeSor.
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| HUSBANDRY | |
|---|---|
| Breeding Considerations |
When maintained as a live colony, animals homozygous for the targeted mutation and hemizygous for the transgene may be intercrossed. Animals homozygous for the transgene are not believed to be viable.
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| Breeding Strategy |
See "Breeding Considerations"
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For Research Use Only.
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