B6.Cg-Pvalb^{tm1.1(cre/ERT2)Hze}/J

Pvalb^{tm1.1(cre/ERT2)Hze}

targeted mutation 1.1

Recombinase-expressing; Inducible

Pvalb

parvalbumin

15

cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene

5461312

Following Tamoxifen induction, Pvalbtm1.1(cre/ERT2)Htz directs reporter gene expression to scattered interneuronal populations in the cortex and hippocampus, as well as to neuronal populations in other brain regions, in a pattern that resembles endogenous Pvalb expression. Additional reporter gene expression is seen in cortical layer 5 neurons, which are unlikely to be interneurons.

(129S6/SvEvTac x C57BL/6NCrl)F1

Embryonic stem (ES) cells, previously targeted with a T2A-Cre vector immediately downstream of the parvalbumin translational STOP codon were re-targeted with a T2A-CreERT2 vector and a FLP-expressing plasmid to facilitate recombination. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site within Pvalb intron 3, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the Pvalb coding sequence, a CreERT2 fusion gene, the Pvalb 3' UTR sequence from exon 4, an attB site, a PGK-hygromycin-SV40polyA cassette and an attP site. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the attB/attP-flanked PGK cassette. The resulting mice were bred with C57BL/6J wildtype mice for at least two additional generations (and the PhiC31 transgene was removed).

C57BL/6J Wild-type from the colony

When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice. The phenotype of homozygous mice has not yet been determined.