STOCK Tg(Jam2-cre/ERT2)2Jrs/J

Cat. No.: CEMM-07250730

Common Name: JAM-B-CreER

The JAM-B-CreER transgene has the junction adhesion molecule 2 promoter/enhancer regions directing tamoxifen-inducible Cre recombinase expression to OFF direction-selective retinal ganglion cells that respond to upward/dorsal motion (J-RGCs). These mice may be useful for tamoxifen-inducible recombination of lox-flanked (floxed) alleles in studying photoreceptors and retinal circuitry.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(Jam2-cre/ERT2)2Jrs show more close
Allele Name	transgene insertion 2 show more close
Allele Attributes	Recombinase-expressing; Inducible show more close
Gene Symbol	Tg(Jam2-cre/ERT2)2Jrs show more close
Gene Name	transgene insertion 2 show more close
Chromosome	UN show more close
Expressed Genes	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene show more close
MGI Accession ID	3785734 show more close
Site of Expression	Tamoxifen induction results in Cre recombinase activity in OFF direction-selective retinal ganglion cells that respond to upward/dorsal motion. show more close
Strain of Origin	Not Specified show more close
Molecular Note	A tamoxifen-inducible form of Cre recombinase and a FRT lanked neomycin selection cassette was put under the control of Jam2 promoter sequences. Transgenic founder mice were crossed with Flpe transgenic mice to remove the neomycin selection cassette. Expression of Cre was confirmed in a subset of retinal neurons upon tamoxifen induction. show more close

HUSBANDRY
Suggested Controls	C57BL/6J show more close
Breeding Considerations	When maintaining a live colony, hemizygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. The donating investigator reports that homozygous and hemizygous mice are viable and fertile with no reported phenotype abnormalities to date - although homozygous mice may be smaller (see genotyping note below). Genotyping note: In 2017, it is the experience that breeding JAM-B-CreER BAC transgene hemizygous mice together only produces offspring that genotype (via Cre qPCR) as hemizygous or non-carrier - no homozygotes are observed. This suggests that in our colony, homozygous mice may not be viable, and may also indicate a single copy of the transgene has integrated into the genome. show more close

HUSBANDRY

Suggested Controls

C57BL/6J

show more close

Breeding Considerations

When maintaining a live colony, hemizygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. The donating investigator reports that homozygous and hemizygous mice are viable and fertile with no reported phenotype abnormalities to date - although homozygous mice may be smaller (see genotyping note below). Genotyping note: In 2017, it is the experience that breeding JAM-B-CreER BAC transgene hemizygous mice together only produces offspring that genotype (via Cre qPCR) as hemizygous or non-carrier - no homozygotes are observed. This suggests that in our colony, homozygous mice may not be viable, and may also indicate a single copy of the transgene has integrated into the genome.

show more close

For Research Use Only.