STOCK Tg(Slc17a8-icre)1Edw/SealJ

Tg(Slc17a8-icre)1Edw

transgene insertion 1

Recombinase-expressing

Tg(Slc17a8-icre)1Edw

transgene insertion 1

UN

Cre, Cre recombinase, bacteriophage P1

5316477

iCre expression is seen in interneurons in the hippocampus, cortex, striatum, and cerebellum, with some expression seen in amacrine cells of the inner retina.

FVB/N

The C57BL/6 mouse bacterial artificial chromosome (BAC) RP24-88H21, containing only the Slc17a8locus, was modified by targeting a codon optimized Cre recombinase (iCre), followed by an SV40 polyA signal, into the ATG start site of theSlc17a8 locus. The modified BAC was microinjected into fertilized FVB/N oocytes and transgenic founders were obtained and crossed to mice expressing a Cre-dependent reporter. Activity of iCre is seen in interneurons in the hippocampus, cortex, striatum, and cerebellum, with some expression seen in amacrine cells of the inner retina. Two of four founder lines expressed Cre, and one line was analyzed further.

Noncarrier

When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6 inbred mice. For Cre-lox experiments and to reduce the frequency of germline deletion of the floxed allele, researchers may consider maintaining the VGluT3-Cre transgene as hemizygous rather than homozygous. Researchers are urged to monitor their Cre;floxed double mutant mice for any undesirable germline deletion of the floxed allele (e.g. genotyping tail/ear samples). Researchers may consider also maintaining a VGluT3-Cre colony separate from any floxed colonies.

Noncarrier x Hemizygote; Hemizygote x Noncarrier