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C57BL/6N-Gt(ROSA)26Sortm1(CAG-GCaMP6f)Khakh/J
Cat. No.: CEMM-07250743
Common Name: R26-Lck-GCaMP6fflox
The Lck-GCaMP6fflox (or R26-Lck-GCaMP6fflox) knock-in mice have Cre-dependent expression of a plasma membrane-targeted GCaMP6f. Lck-GCaMP6f is a detector of single neuronal action potentials with fast response kinetics. It has an Lck-tag to improve its ability to reveal calcium signals in near membrane regions and fine processes (e.g., astrocyte processes). After Cre exposure, bright GCaMP6f fluorescence is observed following calcium binding (such as neuronal activation).
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-GCaMP6f)Khakh
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will express EGFP fluorescence in the Cre-expressing tissues following calcium binding (such as neuronal activation).
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| Strain of Origin |
C57BL/6N
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| Molecular Note |
The targeting vector is designed with (from 5' to 3') with a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), an FRT site, a loxP-flanked STOP cassette, the GCaMP6f coding sequence with N-terminal Lck tag (described below),a WPRE, a BGH polyA signal, and an attB/attP-flanked PGK-FRT Neo-polyA cassette. Lck-GCaMP6f is a GCaMP6 fast variant calcium indicator with an N-terminal Lck-tag that targets GCaMP6f to the plasma membrane. In the absence of calcium binding, dim EGFP fluorescence is expected as there is a pore from the outside of its barrel into the chromophore. Upon calcium binding, this pore becomes occupied and fluorescence is inCreased.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6NJ
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| Breeding Considerations |
Heterozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. It is the experience of CD BioScience that homozygous mice are viable and fertile. When maintaining a live colony, heterozygous mice may be bred with wildtype mice from the colony or with C57BL/6NJ inbred mice.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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