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B6;129S-Gt(ROSA)26Sortm1(CAG-COX8A/Dendra2)Dcc/J
Cat. No.: CEMM-07250398
Common Name: PhAM floxed
PhAMfloxed (photo-activatable mitochondria) mice conditionally express a mitochondrial-specific version of Dendra2 green/red photoswitchable monomeric fluorescent protein. Cre excision of a floxed stop segment permits expression of green fluorescence in mitochondria. Exposure of green fluorescent mitochondria to 405 nm laser light irreversibly switches green fluorescence to red, enabling measurements of mitochondrial fusion and transport.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-COX8A/Dendra2)Dcc
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
Dendra, Dendra photoswitchable fluorescent protein, octocoral Dendronephthya
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| MGI Accession ID | show more close |
| Site of Expression |
Cre excision of the stop signal results in expression of Dendra2, a green-to-red irreversibly photo-switchable fluorescent protein, in the mitochondria of Cre-expressing tissues.
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| Strain of Origin |
129S6/SvEvTac
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| Molecular Note |
Dendra2 (a green to red irreversibly photoswitchable monomeric fluorescent protein derived from octocoral Dendronephthya sp.) fused to the mitochondrial targeting signal of subunit VIII of cytochrome c oxidase (mito-Dendra2), bovine growth hormone (bGH) polyadenylation signal, and an FRT flanked neomycin cassette were introduced to Gt(ROSA)26Sor downstream of a CAG (CMV-beta-actin) promoter and loxP-flanked termination signal.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Heterozygotes are viable and fertile. Homozygous matings have been unproductive in CD BioSciences colony.
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| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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