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B6.129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
Cat. No.: CEMM-07250037
Common Name: MADM-GR
These knock-in mice were developed to utilize the mosaic analysis with double markers (MADM) system that allows simultaneous labeling and gene knockout in clones of somatic cells or isolated single cells in vivo. This strain carries the green fluorescent protein, EGFP (G), and the red fluorescent protein, Dsred2 (R), as markers to be used in conjunction with strains carrying the reciprocal chimeric marker genes (MADM-RG).
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo
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| Allele Name |
targeted mutation 3
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| Allele Attributes |
Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral
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| MGI Accession ID | show more close |
| Site of Expression |
When crossed to reciprocal strain and then to a Cre strain, differential expression of the reporter protein(s) will be expressed in daughter cells, depending on chromatid segregation
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| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
A construct containing the CMV beta-actin enhancer/promoter, SV40 poly(A) signal, and encoding the N-terminal part of EGFP and C-terminal part of Dsred2 (red fluorescent protein; also tagged with 6 copies of the MYC epitope) separated by a beta-globin intron containing a loxP-flanked neomycin resistance gene, was knocked into ES cells at the ROSA26 locus. No protein is expressed from the chimeric locus in absence of Cre-mediated recombination because the coding sequences are interrupted by the intron in different reading frames. After recombination with the reciprocal mutation at the same locus carried by another mouse line, which restores the reading frames of EGFP and RFP, RNA splicing will remove the loxP sites, resulting in functional protein expression.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6.129
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| Breeding Considerations |
Homozygous females on a 129 genetic background have been reported to be subfertile. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice.
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For Research Use Only.
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