STOCK Tg(Six3-cre)69Frty/GcoJ

Cat. No.: CEMM-07250423

Common Name: Six3A1A2-Cre

Six3Cre mice express Cre recombinase in the developing retina and forebrain starting at E9.0.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(Six3-cre)69Frty show more close
Allele Name	transgene insertion 69 show more close
Allele Attributes	Recombinase-expressing show more close
Gene Symbol	Tg(Six3-cre)69Frty show more close
Gene Name	transgene insertion 69 show more close
Chromosome	UN show more close
Expressed Genes	Cre, Cre recombinase, bacteriophage P1 show more close
MGI Accession ID	3574771 show more close
Site of Expression	Cre recombinase is expressed in the developing retina and forebrain. show more close
Strain of Origin	(C57BL/6 x DBA/2)F2 show more close
Molecular Note	Cre cDNA with an SV40 NLS and bovine growth hormone pA were placed under the control of the Six3 regulatory sequences by insertion into the first coding exon. This line represents lines 22, 46, and 51. show more close

HUSBANDRY
Suggested Controls	Noncarrier show more close
Breeding Considerations	When maintaining a live colony, heterozygous mice may be bred together. The donating investigator has not attempted to make a homozygous colony. For Cre-lox experiments and to reduce the frequency of germline deletion of the floxed allele, researchers may consider maintaining the Six3-Cre transgene as hemizygous rather than homozygous and in males instead of females. Researchers are urged to monitor their Cre;floxed double mutant mice for any undesirable germline deletion of the floxed allele (e.g. genotyping tail/ear samples). Researchers may consider also maintaining a Six3-Cre colony separate from any floxed colonies. See Detailed Description for more details. show more close

HUSBANDRY

Suggested Controls

Noncarrier

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Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together. The donating investigator has not attempted to make a homozygous colony. For Cre-lox experiments and to reduce the frequency of germline deletion of the floxed allele, researchers may consider maintaining the Six3-Cre transgene as hemizygous rather than homozygous and in males instead of females. Researchers are urged to monitor their Cre;floxed double mutant mice for any undesirable germline deletion of the floxed allele (e.g. genotyping tail/ear samples). Researchers may consider also maintaining a Six3-Cre colony separate from any floxed colonies. See Detailed Description for more details.

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For Research Use Only.