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B6;129S6-Gt(ROSA)26Sortm1Acoh/J
Cat. No.: CEMM-07250690
Common Name: Floxopatch; Optopatch2; Ai130
Floxopatch (also called Optopatch) mice are a sensitive, fast optogenetic tool for targeted, simultaneous optical perturbation and measurement of membrane voltage. Animals express variants of a near-infrared archaerhodopsin-based voltage indicator (QuasAr2-dark mOrange2; QuasAr2 is near infrared fluorescent, dark mOrange2 is non-fluorescent) and a blue light-gated channelrhodopsin actuator (CheRiff-EGFP; green fluorescent) in the cell plasma membrane after cre-mediated excision of a floxed Stop cassette.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1Acoh
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
RFP, Red Fluorescent Protein, coral; GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
Neurons from Floxopatch mice crossed with a variety of Cre driver lines report spontaneous and optically-evoked activity in vitro, in acute brain slice, and in vivo in somatosensory ganglia.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
A cassette containing pCAG-FRT loxp-Stop codons-3x SV40 polyA-loxP-Optopatch2-WPRE (woodchuck hepatitis post-transcriptional regulatory element)-bGHpA (bovine growth hormone polyadenylation signal)-AttB-pPGK-FRT neo-pA-AttP was introduced to intron 1. Optopatch2 is bicistronic construct that coexpresses QuasAr2-dark mOrange2 (an enhanced archaerhodopsin-3 (Arch)-based voltage indicator) and CheRiff-EGFP (a sensitive blue-shifted optogenetic actuator) separated by the porcine teschovirus-1 (P2A) peptide. This peptide causes a ribosomal skip, leading to near-balanced co-expression of the QuasAr2-dark mOrange2 and CheRiff-EGFP upon Cre-mediated excision of the floxed Stop cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6NJ
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| Breeding Considerations |
Homozygotes and heterozygotes are viable and fertile.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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