B6.Cg-Tg(CAG-GFP, -EIF4EBP1*)2969Als/J

Cat. No.: CEMM-07250754

Common Name: Tg-4EBP1mt

The Tg-4EBP1mt transgene has a floxed-GFP-3xSTOP cassette preventing expression of constitutively active, mTORC1-nonresponsive mutant variant of human EIF4EBP1. These mice may be useful for Cre recombinase-inducible studies involving mTOR complex 1 signaling and metabolism.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(CAG-GFP,-EIF4EBP1*)2969Als show more close
Allele Name	transgene insertion 2969 show more close
Allele Attributes	Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence; Humanized sequence show more close
Gene Symbol	Tg(CAG-GFP,-EIF4EBP1*)2969Als show more close
Gene Name	transgene insertion 2969 show more close
Chromosome	UN show more close
Expressed Genes	GFP, Green Fluorescent Protein; EIF4EBP1, eukaryotic translation initiation factor 4E binding protein 1, human show more close
MGI Accession ID	5896791 show more close
Site of Expression	GFP has widespread expression in the absence of Cre. show more close
Strain of Origin	Not Specified show more close
Molecular Note	The transgene has (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from CAGGS), a loxP-GFP-3xSTOP-loxP cassette and a cDNA sequence encoding human EIF4EBP1 rendered constitutively active and mTORC1-nonresponsive by threonine-to-alanine mutations at two mTOR phosphorylation sites [T37A and T46A]). In the absence of Cre recombinase, mice have widespread expression of GFP, and no human EIF4EBP1 expression. show more close

HUSBANDRY
Suggested Controls	C57BL/6J show more close
Breeding Considerations	When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6J inbred mice. it has not been attempted to make this strain homozygous. show more close
Breeding Strategy	Noncarrier x Hemizygote; Hemizygote x Noncarrier show more close

For Research Use Only.