STOCK Tg(Pcp2-cre)1Amc/J

Cat. No.: CEMM-07250044

Common Name: L7Cre

In these transgenic mice the L7/Pcp2 modified promoter directs tissue specific expression of cre in cerebellar Purkinje cells. A co-integrated GFP transgene results in GFP expression in the cerebellum.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(Pcp2-cre)1Amc show more close
Allele Name	transgene insertion 1 show more close
Allele Attributes	Recombinase-expressing show more close
Gene Symbol	Tg(Pcp2-cre)1Amc show more close
Gene Name	transgene insertion 1 show more close
Chromosome	UN show more close
Expressed Genes	Cre, Cre recombinase, bacteriophage P1 show more close
MGI Accession ID	3656031 show more close
Site of Expression	Parasagittal domains of the cerebellum beginning at embryonic day 17 (E17); at E19, low level expression is observed in the most rostral lobe of cerebellum and expression broadens until all Purkinje cells express the transgene in adult mice. show more close
Strain of Origin	Black Swiss show more close
Molecular Note	The transgene was generated by placing the L7-deltaAUG promoter enhancer (from the L7-deltaAUG vector in which all upstream ATGs have been mutated) upstream of Cre in the pSP-13Cre plasmid. The L7/Pcp2 modified promoter can direct tissue specific expression of genes downstream of it but translation can only be initiated from the ATG of the Cre gene downstream of the promoter, resulting in tissue specific expression of Cre protein in cerebellar Purkinje cells. (NOTE: A GFP transgene co-integrated with the Tg(Pcp2-Cre)1Amc transgene. As such, GFP expression is reported in the cerebellum [personal communication]) show more close

HUSBANDRY
Suggested Controls	Noncarrier show more close
Breeding Considerations	When maintaining a live colony, transgenic mice are bred together or to wildtype siblings. show more close

For Research Use Only.