Online Inquiry
FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
Cat. No.: CEMM-07250021
This strain serves as a Cre reporter strain. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision is indicated by luciferase expression in Cre-expressing tissues.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(Luc)Kael
show more
close
|
| Allele Name |
targeted mutation 1
show more
close
|
| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
show more
close
|
| Gene Symbol |
Gt(ROSA)26Sor
show more
close
|
| Gene Name |
gene trap ROSA 26
show more
close
|
| Chromosome |
6
show more
close
|
| Expressed Genes |
luc, luciferase, firefly
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
Cre recombinase inducible expression of luciferase
show more
close
|
| Strain of Origin |
129S6/SvEvTac
show more
close
|
| Molecular Note |
The endogenous locus is interrupted by a construct comprising a loxP site, a neomycin resistance cassette with a strong, triple SV40 polyadenylation signal, and a second loxP site in the same orientation as the first followed by the cDNA sequence encoding firefly luciferase and the bovine growth hormone polyadenylation signal. In cells in which Cre recombinase is expressed, the DNA between the loxP sites is excised, along with one loxP site, permitting expression of the luciferase gene under control of the ubiquitous ROSA26 promoter.
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
FVB/NJ
show more
close
|
| Breeding Considerations |
This strain is maintained as a homozygote.
show more
close
|
| Breeding Strategy |
Homozygote x Homozygote
show more
close
|
For Research Use Only.
Related Products