STOCK Tg(ACTB-tTA2, -MAPT/lacZ)1Luo/J

Tg(ACTB-tTA2,-MAPT/lacZ)1Luo

transgene insertion 1

Reporter; Transactivator

Tg(ACTB-tTA2,-MAPT/lacZ)1Luo

transgene insertion 1

UN

lacZ, beta-galactosidase, E. coli; tTA, tetracycline-controlled transactivator, E. coli; MAPT, microtubule associated protein tau, human

4867698

These CAG-stop-tTA2 transgenic mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline [dox]).

DBA/2

The ii-CAG-stop-tTA2-IRES-tau-lacZ-ii transgene was designed with the human cytomegalovirus (CMV) enhancer/chicken beta-actin core promoter (pCA), a loxP-flanked neomycin resistance gene (neor) with transcriptional STOP cassette, a modified tetracycline-regulated transactivator gene (tTA2), an internal ribosome entry site (IRES), and a bovine microtubule-associated protein tau with beta-galactosidase fusion protein (tau-lacZ). The transgene was further modified to have two copies of a ~250 bp chicken beta-globin HS4 insulator sequence (separated by an ApaLI restriction site) on each side of the transgene. The 5' insulator pair is flanked by Saw1 and Kpn1 restriction sites, while the 3' insulator pair is flanked by Spe1 and Asc1 restriction sites.

Noncarrier

When maintaining a live colony, hemizygous mice may be bred together, to wildtype (non-carrier) mice from the colony, or to C57BL/6J inbred mice. The donating investigator reports that homozygous mice are viable and fertile, and breeds homozygous mice together.